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KASP genotyping validation kit user guide. This enables the user to trial KASP chemistry in their own laboratory. DNA samples, KASP. The thermal cycle is performed and the plate read on the plate reader. 4 or any queries on this manual or running KASP reactions in. Read online KASP genotyping chemistry User guide and manual book pdf free download link book now. All books are in clear copy here, and all files are secure so don't worry about it. This site is like a library, you could find million book here by using search box in the header. Www.lgcgenomics.com KASP - User guide and manual 3 1.
3 www.lgcgenomics.com KASP - User guide and manual 1. Introduction The Kompetitive Allele Specific PCR genotyping system (KASPTM ) is a homogeneous, fluorescent, endpoint genotyping technology. KASP offers the simplest, most cost-effective and flexible way to determine both SNP and insertion/deletion genotypes. Analysis can be carried out in 96-, 384- and 1536-well plate formats. The KASP genotyping system is comprised of two components: 1. The SNP specific KASP Assay mix, separately purchased as a KASP by Design (KBD) or KASP on Demand (KOD) assay (http://www.lgcgenomics.com/genotyping/kasp-genotyping-reagents/kasp-assays -kbd-kod/) 2.
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Kasp Genotyping Chemistry User Guide And Manual Free
The universal KASP Master mix, which contains all other required components including the universal fluorescent reporting system and a specially developed Taq polymerase (http://www.lgcgenomics.com/genotyping/kasp-genotyping-reagents/). The KASP genotyping system has been used successfully in a wide variety of organisms, achieving well over 90% SNP to assay conversion rate.
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2. Principal of the KASP genotyping assay Refer to the website for full animation. http://www.lgcgenomics.com/genotyping/kasp-genotyping-reagents/how-does-kas p-work/ KASP Assay mix KASP Master mix Sample DNA KASP genotyping reaction Traktor pro 2 review.